{"product_id":"3-3-5-5-tetramethylbenzidine-tmb-cas-54827-17-7","title":"3,3′,5,5′-Tetramethylbenzidine (TMB) | CAS 54827-17-7 | ≥98%","description":"\u003cdiv style=\"margin-bottom: 28px;\"\u003e\n\u003cdiv style=\"background: #002147; padding: 10px 20px; margin-bottom: 0;\"\u003e\u003ch3 style=\"margin: 0; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 0.70em; font-weight: bold; letter-spacing: 0.12em; text-transform: uppercase; color: #fff;\"\u003eTechnical Specifications\u003c\/h3\u003e\u003c\/div\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 0.95em; border: 1px solid #e4e8f0; border-top: none; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eCAS Number\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e54827-17-7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eEC \/ EINECS Number\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e259-364-6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eMDL Number\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eMFCD00007748\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eRTECS Number\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eDV2300000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eSMILES\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0; word-break: break-all;\"\u003eCC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eInChI\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0; word-break: break-all;\"\u003eInChI=1S\/C16H20N2\/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14\/h5-8H,17-18H2,1-4H3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eInChIKey\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0; word-break: break-all;\"\u003eUAIUNKRWKOVEES-UHFFFAOYSA-N\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003ePubChem CID\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e41206\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eMolecular Formula\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eC₁₆H₂₀N₂\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eMolecular Weight\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e240.35 g\/mol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eMelting Point\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e167–170 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eSolubility\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eSoluble in ethyl acetate, ethanol, acetone, chloroform, toluene and DMSO. Insoluble in water.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003ePurity\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e≥98%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003ePhysical Form\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eLight cream powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eHS Code\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e2921.59\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eShelf Life\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eRetest period: 36 months from date of manufacture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eStorage Conditions\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333;\"\u003eStore in a cool, dry and dark place in a tightly sealed container under an inert atmosphere. Protect from light and moisture.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 28px;\"\u003e\n\u003cdiv style=\"background: #002147; padding: 10px 20px; margin-bottom: 0;\"\u003e\u003ch3 style=\"margin: 0; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 0.70em; font-weight: bold; letter-spacing: 0.12em; text-transform: uppercase; color: #fff;\"\u003eProduct Description \u0026amp; Scientific Applications\u003c\/h3\u003e\u003c\/div\u003e\n\u003cdiv style=\"padding: 16px 20px; border: 1px solid #e4e8f0; border-top: none; font-family: Georgia, 'Times New Roman', serif; font-size: 0.95em; line-height: 1.65; color: #333;\"\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cstrong\u003e3,3′,5,5′-Tetramethylbenzidine\u003c\/strong\u003e (TMB) is a benzidine with a methyl group on each side of both amino groups. It is a chromogenic hydrogen donor for peroxidases: horseradish peroxidase (HRP), myeloperoxidase and haemoglobin oxidise it with hydrogen peroxide to coloured products.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe colour reaction.\u003c\/strong\u003e Peroxidase removes one electron from TMB to give a radical cation. The first coloured product is a blue charge-transfer complex of TMB with its two-electron oxidation product, the diimine. When the reaction is stopped with sulfuric acid, the solution turns yellow, and the yellow product is the diimine. In the aqueous assay solution, the blue product is read at about 650 nm (or at 370 nm), and the acid-stopped yellow solution at 450 nm.\u003c\/p\u003e\n\u003cdiv style=\"margin:8px 0 18px 0;\"\u003e\n\u003cdiv style=\"display:flex;flex-wrap:wrap;align-items:center;justify-content:flex-start;gap:2px;\"\u003e\n\u003cdiv style=\"display:flex;align-items:center;\"\u003e\u003csvg role=\"img\" style=\"max-width:100%;height:auto;\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" fill=\"none\" stroke=\"#000\" stroke-width=\"1.6\" width=\"210px\" height=\"105px\" viewbox=\"0 0 210 105\"\u003e\u003cpath d=\"M 51.3,89.1 L 61.9,70.7\"\u003e\u003c\/path\u003e\u003cpath d=\"M 61.9,70.7 L 83.1,70.7\"\u003e\u003c\/path\u003e\u003cpath d=\"M 83.1,70.7 L 93.7,52.3\"\u003e\u003c\/path\u003e\u003cpath d=\"M 81.3,67.5 L 90.0,52.3\"\u003e\u003c\/path\u003e\u003cpath d=\"M 93.7,52.3 L 83.1,33.9\"\u003e\u003c\/path\u003e\u003cpath d=\"M 83.1,33.9 L 61.9,33.9\"\u003e\u003c\/path\u003e\u003cpath d=\"M 81.3,37.1 L 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61.9,70.7\"\u003e\u003c\/path\u003e\u003cpath d=\"M 54.9,52.3 L 63.7,67.5\"\u003e\u003c\/path\u003e\u003cpath d=\"M 125.6,70.7 L 115.0,52.3\"\u003e\u003c\/path\u003e\u003cpath d=\"M 127.4,67.5 L 118.6,52.3\"\u003e\u003c\/path\u003e\u003cpath d=\"M 82.0,70.7 L 83.1,70.7 L 83.6,69.8\"\u003e\u003c\/path\u003e\u003cpath d=\"M 83.6,34.8 L 83.1,33.9 L 82.0,33.9\"\u003e\u003c\/path\u003e\u003cpath d=\"M 125.0,34.8 L 125.6,33.9 L 126.6,33.9\"\u003e\u003c\/path\u003e\u003cpath d=\"M 126.6,70.7 L 125.6,70.7 L 125.0,69.8\"\u003e\u003c\/path\u003e\u003cg style=\"font:12px sans-serif;fill:#000;stroke:none\"\u003e\u003ctext x=\"33.6\" y=\"56.5\" text-anchor=\"end\"\u003eH₂N\u003c\/text\u003e\u003ctext x=\"175.1\" y=\"56.5\" text-anchor=\"start\"\u003eNH₂\u003c\/text\u003e\u003c\/g\u003e\u003c\/svg\u003e\u003c\/div\u003e\n\u003cdiv style=\"display:flex;align-items:center;\"\u003e\u003cdiv style=\"display:flex;flex-direction:column;align-items:center;justify-content:center;min-width:90px;max-width:150px;padding:0 4px;text-align:center;font-size:12px;line-height:1.3;\"\u003e\n\u003cdiv\u003eHRP, H₂O₂\u003c\/div\u003e\n\u003cdiv style=\"font-size:30px;line-height:1;\"\u003e⟶\u003c\/div\u003e\n\u003cdiv\u003etwo-electron oxidation\u003c\/div\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003cdiv style=\"display:flex;align-items:center;\"\u003e\u003csvg role=\"img\" style=\"max-width:100%;height:auto;\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" fill=\"none\" stroke=\"#000\" stroke-width=\"1.6\" width=\"210px\" height=\"105px\" viewbox=\"0 0 210 105\"\u003e\u003cpath d=\"M 161.0,13.2 L 149.7,32.8\"\u003e\u003c\/path\u003e\u003cpath d=\"M 149.7,32.8 L 127.2,32.8\"\u003e\u003c\/path\u003e\u003cpath d=\"M 147.8,36.1 L 129.1,36.1\"\u003e\u003c\/path\u003e\u003cpath d=\"M 127.2,32.8 L 115.9,52.3\"\u003e\u003c\/path\u003e\u003cpath d=\"M 115.9,52.3 L 93.3,52.3\"\u003e\u003c\/path\u003e\u003cpath d=\"M 113.9,48.9 L 95.3,48.9\"\u003e\u003c\/path\u003e\u003cpath d=\"M 93.3,52.3 L 82.0,71.8\"\u003e\u003c\/path\u003e\u003cpath d=\"M 82.0,71.8 L 59.5,71.8\"\u003e\u003c\/path\u003e\u003cpath d=\"M 80.1,68.5 L 61.4,68.5\"\u003e\u003c\/path\u003e\u003cpath d=\"M 59.5,71.8 L 48.2,91.4\"\u003e\u003c\/path\u003e\u003cpath d=\"M 59.5,71.8 L 48.2,52.3\"\u003e\u003c\/path\u003e\u003cpath d=\"M 49.2,50.6 L 29.6,50.6\"\u003e\u003c\/path\u003e\u003cpath d=\"M 49.2,54.0 L 29.6,54.0\"\u003e\u003c\/path\u003e\u003cpath d=\"M 48.2,52.3 L 59.5,32.8\"\u003e\u003c\/path\u003e\u003cpath d=\"M 59.5,32.8 L 48.2,13.2\"\u003e\u003c\/path\u003e\u003cpath d=\"M 59.5,32.8 L 82.0,32.8\"\u003e\u003c\/path\u003e\u003cpath d=\"M 61.4,36.1 L 80.1,36.1\"\u003e\u003c\/path\u003e\u003cpath d=\"M 115.9,52.3 L 127.2,71.8\"\u003e\u003c\/path\u003e\u003cpath d=\"M 127.2,71.8 L 149.7,71.8\"\u003e\u003c\/path\u003e\u003cpath d=\"M 129.1,68.5 L 147.8,68.5\"\u003e\u003c\/path\u003e\u003cpath d=\"M 149.7,71.8 L 161.0,91.4\"\u003e\u003c\/path\u003e\u003cpath d=\"M 149.7,71.8 L 161.0,52.3\"\u003e\u003c\/path\u003e\u003cpath d=\"M 160.1,54.0 L 179.6,54.0\"\u003e\u003c\/path\u003e\u003cpath d=\"M 160.1,50.6 L 179.6,50.6\"\u003e\u003c\/path\u003e\u003cpath d=\"M 161.0,52.3 L 149.7,32.8\"\u003e\u003c\/path\u003e\u003cpath d=\"M 82.0,32.8 L 93.3,52.3\"\u003e\u003c\/path\u003e\u003cpath d=\"M 128.3,32.8 L 127.2,32.8 L 126.6,33.7\"\u003e\u003c\/path\u003e\u003cpath d=\"M 82.6,70.9 L 82.0,71.8 L 80.9,71.8\"\u003e\u003c\/path\u003e\u003cpath d=\"M 48.8,53.3 L 48.2,52.3 L 48.8,51.3\"\u003e\u003c\/path\u003e\u003cpath d=\"M 80.9,32.8 L 82.0,32.8 L 82.6,33.7\"\u003e\u003c\/path\u003e\u003cpath d=\"M 126.6,70.9 L 127.2,71.8 L 128.3,71.8\"\u003e\u003c\/path\u003e\u003cpath d=\"M 160.5,53.3 L 161.0,52.3 L 160.5,51.3\"\u003e\u003c\/path\u003e\u003cg style=\"font:12px sans-serif;fill:#000;stroke:none\"\u003e\u003ctext x=\"29.2\" y=\"56.5\" text-anchor=\"end\"\u003eHN\u003c\/text\u003e\u003ctext x=\"180.0\" y=\"56.5\" text-anchor=\"start\"\u003eNH\u003c\/text\u003e\u003c\/g\u003e\u003c\/svg\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:12px;margin:4px 0 0 0;\"\u003e\u003cem\u003ePeroxidase oxidises TMB (left) to the diimine (right). The blue colour of a running assay comes from a charge-transfer complex of TMB and the diimine; after the acid stop, the yellow diimine is read at 450 nm.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eELISA and immunodetection.\u003c\/strong\u003e In enzyme-linked immunosorbent assays (ELISA), TMB detects HRP-labelled antibodies. After the plate is washed, TMB and hydrogen peroxide are added, the bound HRP develops the blue colour, and the reaction is stopped with acid and read at 450 nm. Reading at two wavelengths has been used to extend the measuring range of TMB-based ELISA. TMB is also used as an HRP substrate in staining procedures, including immunohistochemistry.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOther uses.\u003c\/strong\u003e TMB was introduced as a substitute for benzidine in the detection of blood, in which haemoglobin acts as the peroxidase. A modified TMB method is also used to measure lipid hydroperoxides.\u003c\/p\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 28px;\"\u003e\n\u003cdiv style=\"background: #002147; padding: 10px 20px; margin-bottom: 0;\"\u003e\u003ch3 style=\"margin: 0; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 0.70em; font-weight: bold; letter-spacing: 0.12em; text-transform: uppercase; color: #fff;\"\u003eShipping Destinations\u003c\/h3\u003e\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e4e8f0; border-top: none; padding: 12px 16px; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 0.95em;\"\u003e\u003cul style=\"margin: 0; padding: 0; list-style: none;\"\u003e\n\u003cli style=\"padding: 5px 0; border-bottom: 1px solid #f0f0f0;\"\u003e\n\u003cspan style=\"display: inline-block; width: 7px; height: 7px; background: #002147; border-radius: 50%; margin-right: 10px; vertical-align: middle;\"\u003e\u003c\/span\u003eEU \u0026amp; UK: Priority delivery, 2–5 business days.\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0; border-bottom: 1px solid #f0f0f0;\"\u003e\n\u003cspan style=\"display: inline-block; width: 7px; height: 7px; background: #002147; border-radius: 50%; margin-right: 10px; vertical-align: middle;\"\u003e\u003c\/span\u003eUnited States (DDP): 3–7 business days, duties and taxes prepaid.\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0; border-bottom: 1px solid #f0f0f0;\"\u003e\n\u003cspan style=\"display: inline-block; width: 7px; height: 7px; background: #002147; border-radius: 50%; margin-right: 10px; vertical-align: middle;\"\u003e\u003c\/span\u003eEFTA Countries (DDP): 3–7 business days, duties and taxes prepaid.\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0; border-bottom: 1px solid #f0f0f0;\"\u003e\n\u003cspan style=\"display: inline-block; width: 7px; height: 7px; background: #002147; border-radius: 50%; margin-right: 10px; vertical-align: middle;\"\u003e\u003c\/span\u003eWorldwide: 7–14 business days, selected locations.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"NorrChemica™","offers":[{"title":"1.0 g","offer_id":55166391026001,"sku":"NOR-54827177-1g","price":69.2,"currency_code":"EUR","in_stock":true},{"title":"5.0 g","offer_id":55166391058769,"sku":"NOR-54827177-5g","price":84.2,"currency_code":"EUR","in_stock":true},{"title":"10 g","offer_id":55166391091537,"sku":"NOR-54827177-10g","price":98.2,"currency_code":"EUR","in_stock":true},{"title":"25 g","offer_id":55166391124305,"sku":"NOR-54827177-25g","price":149.2,"currency_code":"EUR","in_stock":true},{"title":"100 g","offer_id":55166391157073,"sku":"NOR-54827177-100g","price":549.2,"currency_code":"EUR","in_stock":true},{"title":"250 g","offer_id":55371443306833,"sku":"NOR-54827177-250g","price":1029.2,"currency_code":"EUR","in_stock":false},{"title":"500 g","offer_id":55371443339601,"sku":"NOR-54827177-500g","price":1895.0,"currency_code":"EUR","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0954\/6357\/1793\/files\/NorrChemica_TMB.png?v=1791059030","url":"https:\/\/www.norrchemica.com\/products\/3-3-5-5-tetramethylbenzidine-tmb-cas-54827-17-7","provider":"NorrChemica","version":"1.0","type":"link"}