{"product_id":"edta-ethylenediaminetetraacetic-acid-cas-60-00-4","title":"EDTA (Ethylenediaminetetraacetic Acid) | CAS 60-00-4 | ≥98%","description":"\u003cdiv style=\"margin-bottom: 28px;\"\u003e\n\u003cdiv style=\"background: #002147; padding: 10px 20px; margin-bottom: 0;\"\u003e  \u003ch3 style=\"margin: 0; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 0.70em; font-weight: 700; letter-spacing: 0.12em; text-transform: uppercase; color: #fff;\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 0.95em; border: 1px solid #e4e8f0; border-top: none; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eCAS Number\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e60-00-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eEC \/ EINECS Number\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e200-449-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eMDL Number\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eMFCD00003541\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eRTECS Number\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eAH4025000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eSMILES\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0; word-break: break-all;\"\u003eC(CN(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eInChI\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0; word-break: break-all;\"\u003eInChI=1S\/C10H16N2O8\/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20\/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eInChIKey\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0; word-break: break-all;\"\u003eKCXVZYZYPLLWCC-UHFFFAOYSA-N\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003ePubChem CID\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e6049\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eMolecular Formula\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eC₁₀H₁₆N₂O₈\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eMolecular Weight\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e292.24 g\/mol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eMelting Point\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e237–245 °C (dec.)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eSolubility\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eSparingly soluble in water (~0.5 g\/L at 25 °C); soluble in dilute alkali solutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003ePurity\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e≥98%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003ePhysical Form\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eWhite crystalline powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eHS Code\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e2922.49\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eCountry of Origin\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eFinland\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eShelf Life\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eRetest period: 36 months from date of manufacture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eStorage Conditions\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eStore at room temperature in a tightly sealed container, protected from moisture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-bottom: 28px;\"\u003e\n\u003cdiv style=\"background: #002147; padding: 10px 20px; margin-bottom: 0;\"\u003e  \u003ch3 style=\"margin: 0; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 0.70em; font-weight: 700; letter-spacing: 0.12em; text-transform: uppercase; color: #fff;\"\u003eProduct Description \u0026amp; Scientific Applications\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"padding: 16px 20px; border: 1px solid #e4e8f0; border-top: none; font-family: Georgia, 'Times New Roman', serif; font-size: 0.95em; line-height: 1.65; color: #333;\"\u003e\n\u003cp\u003e\u003cstrong\u003eEDTA\u003c\/strong\u003e (ethylenediaminetetraacetic acid; edetic acid; H₄EDTA) is a hexadentate aminopolycarboxylic acid chelator for divalent- and trivalent-metal-ion control in molecular biology, biochemistry, analytical chemistry, cell culture, and sample preparation. In the fully deprotonated Y⁴⁻ form, its two amine nitrogens and four carboxylates wrap a metal centre into octahedral cage-like complexes with high formation constants (log Kf ≈ 10.7 Ca²⁺, 8.7 Mg²⁺, 16.5 Zn²⁺, 18.8 Cu²⁺, 25 Fe³⁺ at 25 °C, I = 0.1 M). It is a hexaprotic weak acid (H₆Y²⁺ when fully protonated), so the conditional stability constant Kf′ depends strongly on the αY⁴⁻ fraction, making working pH central to every titration and molecular-biology buffer. The free acid is poorly soluble and needs alkaline NaOH or KOH to dissolve at working concentrations; the more soluble disodium-dihydrate and tetrasodium salts allow direct preparation. EDTA binds Ca²⁺ and Mg²⁺ with comparable affinity, so where Ca²⁺-selective chelation is needed alongside physiological Mg²⁺, EGTA is preferred for its much larger Ca²⁺\/Mg²⁺ binding-constant differential.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNucleic-acid protection and enzyme control.\u003c\/strong\u003e EDTA is the standard divalent-cation sequestrant in molecular-biology buffers, suppressing Mg²⁺- and Ca²⁺-dependent enzymes — DNases, divalent-metal-dependent RNases, and metalloproteases — by lowering free metal-ion concentration below their catalytic threshold. It is the EDTA in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) for DNA storage and nuclease-sensitive handling, and in TAE (1×: 40 mM Tris-acetate, 1 mM EDTA, pH ≈ 8.3) and TBE (1×: 89 mM Tris, 89 mM borate, 2 mM EDTA, pH ≈ 8.3) for agarose and polyacrylamide electrophoresis. The same role puts it in stop solutions for restriction digests, ligations, and PCR, and in plasmid- and genomic-DNA lysis buffers and RNA-isolation protocols. It is excluded from active PCR and reverse-transcription mixes, however, because it would sequester the Mg²⁺ cofactor polymerases require.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCell dissociation and adhesion control.\u003c\/strong\u003e EDTA weakens Ca²⁺- and Mg²⁺-dependent cell adhesion in mammalian culture. Cadherin cell–cell contacts require extracellular Ca²⁺ for homophilic engagement, and integrin cell–substrate interactions depend on Ca²⁺, Mg²⁺, and Mn²⁺ at the metal-ion-dependent adhesion site (MIDAS); sequestering these cations disrupts both adhesion classes at once. Common formulations pair 0.05% or 0.25% trypsin with ~0.5–1 mM EDTA in Ca²⁺\/Mg²⁺-free balanced salt solution, letting trypsin reach cleavable peptide bonds once contacts are weakened. Enzyme-free EDTA-in-PBS (typically 0.5–5 mM) serves surface-antigen-sensitive work: flow-cytometry sample preparation, primary-cell dissociation where receptor integrity matters, and stem-cell passaging where trypsin exposure must be minimised.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHematology anticoagulant and histology decalcification.\u003c\/strong\u003e Dipotassium and tripotassium EDTA (K₂EDTA, K₃EDTA) are the standard anticoagulant additives in lavender-top blood tubes: chelation of Ca²⁺ blocks the calcium-dependent steps of the coagulation cascade and stabilises blood-cell morphology. EDTA-anticoagulated whole blood is the matrix of choice for complete blood counts, leukocyte differential analysis, peripheral-blood-smear morphology, and flow-cytometric immunophenotyping; spray-dried K₂EDTA is preferred for these tubes because it minimises the liquid-dilution effect of liquid K₃EDTA. In histology, 10% EDTA buffered to pH 7.4 is the preferred decalcifier for bone, teeth, and other mineralised samples when antigenicity and nucleic-acid integrity must be preserved: it removes calcium phosphate gradually at near-neutral pH, unlike formic, nitric, or hydrochloric acids, leaving protein epitopes and DNA\/RNA quality sufficient for immunohistochemistry, in situ hybridisation, and PCR.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComplexometric titration.\u003c\/strong\u003e EDTA is the classical complexometric reagent: its aminopolycarboxylate framework binds almost all di- and trivalent cations as stable, water-soluble 1:1 complexes whose strict stoichiometry is the basis of the method. Because stability is pH-dependent, titrations run in defined buffers with metallochromic indicators — ammonia\/ammonium chloride at pH 10 with Eriochrome Black T for total water hardness, murexide for direct Ca²⁺ titration in the presence of Mg²⁺ — and masking agents to suppress interfering metals. Applications include water-hardness determination, Ca\/Mg quantification in pharmaceutical and food matrices, transition-metal analysis, and metal-ion buffers that hold a defined free-metal concentration for kinetic and equilibrium work, including trace-metal speciation and analytical method development.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFurther applications.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eChelated iron source (Fe-EDTA) in Murashige–Skoog and other plant tissue-culture and hydroponic media.\u003c\/li\u003e\n\u003cli\u003eMetalloprotease and Mg²⁺\/Mn²⁺-dependent phosphatase inhibition in protease-inhibitor cocktails and biochemical sample preparation.\u003c\/li\u003e\n\u003cli\u003eMetal-stripping reagent for IMAC resin regeneration and adventitious-metal removal in chromatography buffers, excluding active metal-affinity steps.\u003c\/li\u003e\n\u003cli\u003eSequestrant in cosmetic, food-research, and formulation chemistry to limit metal-catalysed oxidation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-bottom: 28px;\"\u003e\n\u003cdiv style=\"background: #002147; padding: 10px 20px; margin-bottom: 0;\"\u003e  \u003ch3 style=\"margin: 0; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 0.70em; font-weight: 700; letter-spacing: 0.12em; text-transform: uppercase; color: #fff;\"\u003eShipping Destinations\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e4e8f0; border-top: none; padding: 12px 16px; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 0.95em;\"\u003e\u003cul style=\"margin: 0; padding: 0; list-style: none;\"\u003e\n\u003cli style=\"padding: 5px 0; border-bottom: 1px solid #f0f0f0;\"\u003e\n\u003cspan style=\"display: inline-block; width: 7px; height: 7px; background: #002147; border-radius: 50%; margin-right: 10px; vertical-align: middle;\"\u003e\u003c\/span\u003eEU \u0026amp; UK: Priority delivery, 2–5 business days.\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0; border-bottom: 1px solid #f0f0f0;\"\u003e\n\u003cspan style=\"display: inline-block; width: 7px; height: 7px; background: #002147; border-radius: 50%; margin-right: 10px; vertical-align: middle;\"\u003e\u003c\/span\u003eUnited States (DDP): 3–7 business days, duties and taxes prepaid.\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0; border-bottom: 1px solid #f0f0f0;\"\u003e\n\u003cspan style=\"display: inline-block; width: 7px; height: 7px; background: #002147; border-radius: 50%; margin-right: 10px; vertical-align: middle;\"\u003e\u003c\/span\u003eEFTA Countries (DDP): 3–7 business days, duties and taxes prepaid.\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0; border-bottom: 1px solid #f0f0f0;\"\u003e\n\u003cspan style=\"display: inline-block; width: 7px; height: 7px; background: #002147; border-radius: 50%; margin-right: 10px; vertical-align: middle;\"\u003e\u003c\/span\u003eWorldwide: 7–14 business days, selected locations.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"NorrChemica™","offers":[{"title":"100 g","offer_id":52240704471377,"sku":"NOR-60004-100g","price":27.0,"currency_code":"EUR","in_stock":true},{"title":"250 g","offer_id":52240704504145,"sku":"NOR-60004-250g","price":37.0,"currency_code":"EUR","in_stock":true},{"title":"500 g","offer_id":52240704536913,"sku":"NOR-60004-500g","price":49.0,"currency_code":"EUR","in_stock":true},{"title":"1 kg","offer_id":52240704569681,"sku":"NOR-60004-1kg","price":69.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0954\/6357\/1793\/files\/EDTA_NORRCHEMICA_98.png?v=1781396073","url":"https:\/\/www.norrchemica.com\/products\/edta-ethylenediaminetetraacetic-acid-cas-60-00-4","provider":"NorrChemica","version":"1.0","type":"link"}