{"product_id":"tcep-hcl-cas-51805-45-9-0-97","title":"TCEP-HCl | CAS 51805-45-9 | ≥97%","description":"\u003cdiv style=\"margin-bottom: 28px;\"\u003e\n\u003cdiv style=\"background: #002147; padding: 10px 20px; margin-bottom: 0;\"\u003e  \u003ch3 style=\"margin: 0; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 0.70em; font-weight: 700; letter-spacing: 0.12em; text-transform: uppercase; color: #fff;\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 0.95em; border: 1px solid #e4e8f0; border-top: none; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eCAS Number\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e51805-45-9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eEC \/ EINECS Number\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e629-759-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eMDL Number\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eMFCD00145469\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eSMILES\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0; word-break: break-all;\"\u003eC(CP(CCC(=O)O)CCC(=O)O)C(=O)O.Cl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eInChI\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0; word-break: break-all;\"\u003eInChI=1S\/C9H15O6P.ClH\/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;\/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eInChIKey\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0; word-break: break-all;\"\u003ePBVAJRFEEOIAGW-UHFFFAOYSA-N\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003ePubChem CID\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e2734570\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eMolecular Formula\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eC₉H₁₆ClO₆P\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eMolecular Weight\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e286.64 g\/mol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eMelting Point\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e175-177 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eSolubility\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eFreely soluble in water (~310 g\/L; ~1.08 M); minimally soluble in methanol and ethanol; poorly soluble in non-polar solvents.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003ePurity\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e≥97%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003ePhysical Form\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eWhite to off-white crystalline powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eHS Code\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003e2931.49\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eShelf Life\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eRetest period: 36 months from date of manufacture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 9px 16px; font-weight: 600; color: #002147; width: 200px; border-bottom: 1px solid #e4e8f0; background: #f7f8fb; font-size: 0.95em; vertical-align: top;\"\u003eStorage Conditions\u003c\/td\u003e\n\u003ctd style=\"padding: 9px 16px; color: #333; border-bottom: 1px solid #e4e8f0;\"\u003eStore at 2–8 °C; –20 °C for long-term storage. Keep tightly closed and protected from moisture. The solid is stable in air for several months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-bottom: 28px;\"\u003e\n\u003cdiv style=\"background: #002147; padding: 10px 20px; margin-bottom: 0;\"\u003e  \u003ch3 style=\"margin: 0; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 0.70em; font-weight: 700; letter-spacing: 0.12em; text-transform: uppercase; color: #fff;\"\u003eProduct Description \u0026amp; Scientific Applications\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"padding: 16px 20px; border: 1px solid #e4e8f0; border-top: none; font-family: Georgia, 'Times New Roman', serif; font-size: 0.95em; line-height: 1.65; color: #333;\"\u003e\n\u003cp\u003e\u003cstrong\u003eTCEP·HCl\u003c\/strong\u003e (tris(2-carboxyethyl)phosphine hydrochloride) is a water-soluble trialkylphosphine used to reduce disulfide bonds. In water it reduces disulfides rapidly and completely at mildly acidic pH (around 4.5) and preferentially cleaves the more strained disulfides.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMechanism.\u003c\/strong\u003e Reduction follows the general pathway for disulfide cleavage by phosphines in water: phosphorus attacks the S–S bond and is itself oxidised to the phosphine oxide, converting the disulfide to two free thiols. Because the phosphine is consumed as its oxide, the reduction does not reverse — unlike thiol reductants, which act through a redox equilibrium.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProperties.\u003c\/strong\u003e TCEP is an odourless reducing agent, resistant to air oxidation and stable across a broad pH range. Below pH 8 it is more effective than dithiothreitol (DTT) — which itself oxidises above pH 7.5 — although the better choice between the two reductants is application-specific. Two advantages over DTT are documented in protein biochemistry: nitroxide spin labels are two-to-four times more stable in TCEP than in DTT, and the Ni²⁺ that contaminates proteins eluted from nickel-affinity columns rapidly oxidises DTT but leaves TCEP unaffected.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHandling.\u003c\/strong\u003e As the hydrochloride, TCEP·HCl gives an acidic solution in water and is buffered to the required working pH before use.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications and Uses\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDisulfide reduction in proteins and peptides:\u003c\/strong\u003e cleaves inter- and intrachain disulfides and holds cysteines reduced — the standard reduction step ahead of alkylation, labelling and analysis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eThiol–maleimide bioconjugation:\u003c\/strong\u003e because it need not be removed before maleimide chemistry, TCEP allows reduction and conjugation to run together, streamlining site-specific labelling of cysteine thiols.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMass spectrometry:\u003c\/strong\u003e used for in situ reduction of disulfides in peptides and proteins for MALDI and related mass-spectrometric analysis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eThiolated oligonucleotide immobilisation:\u003c\/strong\u003e reduces the protecting disulfide on thiol-modified DNA, freeing the thiol for attachment; it has been optimised for immobilising thiolated oligonucleotides on electrode surfaces in microfluidic biosensors.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEPR spin labelling:\u003c\/strong\u003e the enhanced stability of nitroxide spin labels in TCEP makes it advantageous for EPR spectroscopy.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-bottom: 28px;\"\u003e\n\u003cdiv style=\"background: #002147; padding: 10px 20px; margin-bottom: 0;\"\u003e  \u003ch3 style=\"margin: 0; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 0.70em; font-weight: 700; letter-spacing: 0.12em; text-transform: uppercase; color: #fff;\"\u003eShipping Destinations\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e4e8f0; border-top: none; padding: 12px 16px; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 0.95em;\"\u003e\u003cul style=\"margin: 0; padding: 0; list-style: none;\"\u003e\u003cli style=\"padding: 5px 0; border-bottom: 1px solid #f0f0f0;\"\u003e\n\u003cspan style=\"display: inline-block; width: 7px; height: 7px; background: #002147; border-radius: 50%; margin-right: 10px; vertical-align: middle;\"\u003e\u003c\/span\u003eEU \u0026amp; UK only: Priority delivery, 2–5 business days. Classified as dangerous goods — shipping restrictions apply outside the EU\/UK.\u003c\/li\u003e\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"NorrChemica™","offers":[{"title":"1 g","offer_id":53965162774865,"sku":"NOR-51805459-1g","price":24.9,"currency_code":"EUR","in_stock":true},{"title":"2 g","offer_id":53965162807633,"sku":"NOR-51805459-2g","price":34.9,"currency_code":"EUR","in_stock":true},{"title":"5 g","offer_id":53965162840401,"sku":"NOR-51805459-5g","price":47.9,"currency_code":"EUR","in_stock":true},{"title":"10 g","offer_id":53965162873169,"sku":"NOR-51805459-10g","price":74.2,"currency_code":"EUR","in_stock":true},{"title":"25 g","offer_id":53965162905937,"sku":"NOR-51805459-25g","price":129.2,"currency_code":"EUR","in_stock":true},{"title":"50 g","offer_id":53965162938705,"sku":"NOR-51805459-50g","price":198.2,"currency_code":"EUR","in_stock":true},{"title":"100 g","offer_id":53965162971473,"sku":"NOR-51805459-100g","price":329.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0954\/6357\/1793\/files\/NorrChemica_TCEP-HCl_v2.png?v=1782918213","url":"https:\/\/www.norrchemica.com\/products\/tcep-hcl-cas-51805-45-9-0-97","provider":"NorrChemica","version":"1.0","type":"link"}