NorrChemica
Alcian Blue 8GX | CAS 33864-99-2 | Dye content ≥ 80%, Certified | Histology & Microscopy Stain
Alcian Blue 8GX | CAS 33864-99-2 | Dye content ≥ 80%, Certified | Histology & Microscopy Stain
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🧪 Technical Specifications
- CAS Number: 33864-99-2
- SMILES: CN(C)C(=[N+])SCC1=CC2=C(C=C1)C3=NC4=NC(=NC5=NC(=NC2=N3)C6=CC(=C(C=C65)CS C(=[N+](C)C)N(C)C)[Cl-])C7=CC(=C(C=C74)CSC(=[N+](C)C)N(C)C)[Cl-].[Cl-].[Cl-]
- InChI: InChI=1S/C56H68N16S4.4ClH/c1-65(2)49(69)41-25-29-33(21-37(41)73-53(65)61-9)45-57-46(34-22-38(42(26-30(34)45)74-54(66(3)4)62-10)50(70)66)59-48(36-24-40(44(28-32(36)59)76-56(68(7)8)64-12)52(72)68)60-47(35-23-39(43(27-31(35)60)75-55(67(5)6)63-11)51(71)67)58-45;;;;/h21-28H,1-12H3,(H-,57,58,59,60,61,62,63,64,69,70,71,72,73,74,75,76);4*1H/q+4;;;;/p-4
- InChIKey: COYIFAYFBNLDEI-UHFFFAOYSA-J
- Molecular Formula: C₅₆H₆₈Cl₄CuN₁₆S₄·xH₂O
- Molecular Weight: 1298.0 g/mol
- Purity: Dye content ≥ 80%, Certified
- Physical Form: Dark blue-greenish powder
- HS Code: 3204.19
- Country of Origin: Finland
- Shelf Life: ≥ 36 months (unopened, recommended storage)
- Storage Conditions: Store tightly closed at room temperature (15–25 °C), dry conditions; protect from moisture and prolonged light.
🔬 Common Scientific Applications
🧫 Histology of acidic mucins/GAGs: Robust stain for acidic mucopolysaccharides (glycosaminoglycans) and acidic mucins in tissues.
⚖️ pH-selective protocols:
pH 2.5: stains carboxylated + sulfated mucins/GAGs.
pH 1.0: preferential for sulfated mucins.
🧪 Critical electrolyte concentration (CEC): MgCl₂/salt titration tunes selectivity among GAG species.
🔵 AB–PAS combo: Alcian Blue–Periodic Acid–Schiff sequence to differentiate acidic (blue/teal) vs neutral (magenta) mucins.
🧴 Cartilage & ECM studies: Quantification/visualization of cartilage matrix GAGs in tissue engineering sections.
🌿 Botany & plant cell walls: Stains acidic pectins and wall polysaccharides (with controlled pH/ionic strength).
🧷 Biomaterials & hydrogels: Mapping/assaying sulfated GAG content in scaffolds, gels, and coatings.
🧫 Mast cells & goblet cells: Identification of granules and goblet cell mucins in selected protocols.
Optical Properties:
🌈 Primary use: Chromogenic (brightfield) stain.
📉 Absorbance (λₘₐₓ): typically ~610–620 nm in aqueous media (method- and matrix-dependent).
✨ Fluorescence: generally weak/negligible under standard conditions; not used as a fluorescence probe.
⚙️ Color tone: blue/blue-green shifts with pH, counter-ions, and fixation.
R&D use only. Results depend on fixation, pH control, electrolyte concentration, and section processing. Verify your protocol and consult the SDS/CoA for lot-specific details.
⚠️ Safety & Transport
- Hazard Class (UN/DG): Not classified as Dangerous Goods (IATA/ADR) — see SDS
- GHS Signal Word: None
- GHS Hazard Statements: None
- GHS Precautionary Statements: P264, P280
- Dangerous Goods Notes: Not regulated for transport as DG. Ship via standard services; include SDS.
🔎 Advanced Identifiers
- PubChem CID: 12965476
Alcian Blue 8GX | CAS 33864-99-2 | Dye content ≥ 80%, Certified | Histology & Microscopy Stain
🧪 The NorrChemica™ Standard
- European Certified Origin: Sourced exclusively from accredited EU manufacturers. Full supply chain traceability.
- Analytical Purity: Identity-verified material. Strictly meets or exceeds defined grade specifications.
- 🇪🇺 Intra-EU Trade: Frictionless, tariff-free shipping within the European Union (Single Market).
- 🌍 Global Support: Full customs documentation provided for UK, Canada, and EFTA orders.
- Professional Logistics: Shipped from Finland via premium tracked couriers (UPS/Posti).
🧰 Packaging & Storage
- Supplied in sealed HDPE or amber glass containers
- Store tightly closed in a cool, dry place
- Shelf life: ≥ 36 months under recommended conditions
- Batch-specific CoA and SDS provided
⚠️ Compliance & Safety
- Not for human or veterinary use.
- Mandatory Safety: Always follow the safety instructions and required Personal Protective Equipment (PPE) as detailed in the Safety Data Sheet (SDS).
- Regulatory Status: The specific classification is product-dependent; see the SDS supplied with your order.
- Documentation: A Certificate of Analysis (CoA) and GHS-compliant SDS are available for every order.
- Payment: To ensure regulatory compliance for specialty chemicals, we process orders via Wise (Bank Transfer) or Manual Invoice.
Shipping & Delivery
We ship from our facility in Finland (EU).
- 🇪🇺 EU & UK: typically 2–5 business days.
- 🌍 Selected destinations (e.g., Canada, Japan, Australia, EFTA): typically 4–12 business days.
- ❌ 🇺🇸 United States: shipping unavailable.
- 🧾 Full documentation (CoA + GHS-compliant SDS) included.
🧬 About NorrChemica™
NorrChemica™ is a Finnish supplier of high-purity specialty reagents and niche research materials. We focus on reliable EU shipping, transparent labeling, and accessible pricing for technical users worldwide.
⚖️ Terms of Sale & Disclaimer Sold strictly for laboratory research, analysis, and technical applications. Not for human consumption or food use. By purchasing this item, you confirm professional or educational intent.
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