NorrChemica
Toluidine Blue O Powder | CAS 92-31-9 | Research Grade ≥ 75.0% Dye Content (3rd-Party tested) | Metachromatic Histology Stain | EU Seller
Toluidine Blue O Powder | CAS 92-31-9 | Research Grade ≥ 75.0% Dye Content (3rd-Party tested) | Metachromatic Histology Stain | EU Seller
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🧪 Technical Specifications
- CAS Number: 92-31-9
- SMILES: CC1=CC2=C(C=C1N)N=C3C=CC(=[N+](C)C)C=C3S2.[Cl-]
- InChI: InChI=1S/C15H16N3S.ClH/c1-10-8-12-14(9-13(10)18-2)19-11-6-7-15(17-3)16-11;/h6-9H,1-5H3;1H/q+1;/p-1
- InChIKey: XFNOTSREUXSADP-UHFFFAOYSA-M
- Molecular Formula: C₁₅H₁₆ClN₃S
- Molecular Weight: 305.82 g/mol
- Purity: Dye content ≥75%
- Physical Form: Blue/violet crystalline powder
- HS Code: 3204.13
- Country of Origin: Finland
- Shelf Life: ≥ 36 months
- Storage Conditions: Store tightly closed at 15–25 °C, in dry conditions, and protected from light (amber container recommended). Keep away from oxidizers and strong acids/bases.
🔬 Common Scientific Applications
🧫 Metachromatic histology stain: Highlights acidic polysaccharides/GAGs (e.g., cartilage matrix, mast-cell granules). Color shifts (blue → purple) indicate metachromasia.
🧠 Nissl & neural tissue staining: Rapid screening of neuron soma/Nissl substance in paraffin or resin sections.
🧴 Resin semi-thin sections: Quick contrast for epoxy/Spurr semi-thin tissue sections before EM work.
⚖️ pH-selective protocols: Low-pH staining biases toward sulfated vs carboxylated groups; tune specificity via pH/ionic strength.
🧪 Critical electrolyte concentration (CEC): Salt (e.g., MgCl₂) titrations adjust selectivity among GAG species.
🌿 Plant anatomy: Stains lignified/suberized cell walls; good general contrast for botanical sections.
🧷 Polyanion mapping & materials: Qualitative detection of polyanionic sites in hydrogels, scaffolds, and ECM-mimetic materials.
⚡ Photochemistry (method development): Can act as a photosensitizer under red light in ROS-generation tests (bench models).
Optical Properties:
🌈 Primary use: Chromogenic (brightfield) stain with metachromasia.
📉 Absorbance (λ_max): typically ~620–630 nm in aqueous media (matrix/pH dependent).
✨ Fluorescence: Weak red fluorescence; can be excited ~620–640 nm (e.g., 633 nm), emitting around ~650–670 nm. Often quenched by aggregation/binding state.
🧪 Notes: Spectral behavior varies with pH, concentration, counter-ions, and substrate binding; verify on your instrument.
R&D use only. Optimize pH/ionic strength for your target (sulfated vs carboxylated) and follow the SDS/CoA for handling.
⚠️ Safety & Transport
- Hazard Class (UN/DG): Not classified as a dangerous good (DG) for transport according to international regulations (IATA/ADR/DOT)
- GHS Signal Word: Warning
- GHS Hazard Statements: H302 - Harmful if swallowed, ; H315 - Causes skin irritation
- GHS Precautionary Statements: P280 (Wear protective gloves/eye protection), P301+P312 (IF SWALLOWED: call a doctor)
- Dangerous Goods Notes: Not classified as a dangerous good (DG) for transport according to international regulations (IATA/ADR/DOT)
🔎 Advanced Identifiers
- PubChem CID: 7069
Toluidine Blue O Powder | CAS 92-31-9 | Research Grade ≥ 75.0% Dye Content (3rd-Party tested) | Metachromatic Histology Stain | EU Seller
🧪 The NorrChemica™ Standard
- European Certified Origin: Sourced exclusively from accredited EU manufacturers. Full supply chain traceability.
- Analytical Purity: Identity-verified material. Strictly meets or exceeds defined grade specifications.
- 🇪🇺 Intra-EU Trade: Frictionless, tariff-free shipping within the European Union (Single Market).
- 🌍 Global Support: Full customs documentation provided for UK, Canada, and EFTA orders.
- Professional Logistics: Shipped from Finland via premium tracked couriers (UPS/Posti).
🧰 Packaging & Storage
- Supplied in sealed HDPE or amber glass containers
- Store tightly closed in a cool, dry place
- Shelf life: ≥ 36 months under recommended conditions
- Batch-specific CoA and SDS provided
⚠️ Compliance & Safety
- Not for human or veterinary use.
- Mandatory Safety: Always follow the safety instructions and required Personal Protective Equipment (PPE) as detailed in the Safety Data Sheet (SDS).
- Regulatory Status: The specific classification is product-dependent; see the SDS supplied with your order.
- Documentation: A Certificate of Analysis (CoA) and GHS-compliant SDS are available for every order.
- Payment: To ensure regulatory compliance for specialty chemicals, we process orders via Wise (Bank Transfer) or Manual Invoice.
Shipping & Delivery
We ship from our facility in Finland (EU).
- 🇪🇺 EU & UK: typically 2–5 business days.
- 🌍 Selected destinations (e.g., Canada, Japan, Australia, EFTA): typically 4–12 business days.
- ❌ 🇺🇸 United States: shipping unavailable.
- 🧾 Full documentation (CoA + GHS-compliant SDS) included.
🧬 About NorrChemica™
NorrChemica™ is a Finnish supplier of high-purity specialty reagents and niche research materials. We focus on reliable EU shipping, transparent labeling, and accessible pricing for technical users worldwide.
⚖️ Terms of Sale & Disclaimer Sold strictly for laboratory research, analysis, and technical applications. Not for human consumption or food use. By purchasing this item, you confirm professional or educational intent.
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