Lab Journal
Coumarins, rhodamines and fluorescein in soluti...
A fluorescent dye can give a weaker signal, or emit at a different wavelength, than its data sheet states. Published absorption and emission maxima, molar absorption coefficients and quantum yields...
Coumarins, rhodamines and fluorescein in soluti...
A fluorescent dye can give a weaker signal, or emit at a different wavelength, than its data sheet states. Published absorption and emission maxima, molar absorption coefficients and quantum yields...
Coupling reagents beyond the amide bond: oxidat...
Peptide coupling reagents are bought to make amides. Seven other reactions they do, from the oxidation of H-phosphonates by COMU to the Lossen rearrangement with CDI: which reagent does which,...
Coupling reagents beyond the amide bond: oxidat...
Peptide coupling reagents are bought to make amides. Seven other reactions they do, from the oxidation of H-phosphonates by COMU to the Lossen rearrangement with CDI: which reagent does which,...
Why a staining solution stops working: hematoxy...
A staining solution that worked last month gives a weak or patchy result today. Six stains, and for each one the fault that most often causes it, what causes the...
Why a staining solution stops working: hematoxy...
A staining solution that worked last month gives a weak or patchy result today. Six stains, and for each one the fault that most often causes it, what causes the...
Dye content on the label: why a stain at 85% is...
A stain labelled "dye content ≥85%" is not 15% impure. The figure is the share of the powder that is coloured compound, and most of the rest is water and...
Dye content on the label: why a stain at 85% is...
A stain labelled "dye content ≥85%" is not 15% impure. The figure is the share of the powder that is coloured compound, and most of the rest is water and...
Radical coupling of carboxylic acids and boroni...
Neutral boron + fluoride from TMAF + an electrode = new radical chemistry. A paper in Nature Chemistry (5 October 2026) joins alkyl carboxylic acids to alkylboronic acids by electrolysis,...
Radical coupling of carboxylic acids and boroni...
Neutral boron + fluoride from TMAF + an electrode = new radical chemistry. A paper in Nature Chemistry (5 October 2026) joins alkyl carboxylic acids to alkylboronic acids by electrolysis,...
Choosing a Coupling Reagent for Amide and Pepti...
Two amide couplings that look identical on paper can give a clean product or a racemised mess, depending only on the reagent and additive. A practical guide to the carbodiimides,...
Choosing a Coupling Reagent for Amide and Pepti...
Two amide couplings that look identical on paper can give a clean product or a racemised mess, depending only on the reagent and additive. A practical guide to the carbodiimides,...