• Chemical structure of 5-Methylcytidine (C₁₀H₁₅N₃O₅), CAS 2140-61-6, ≥97% — modified nucleoside (m5C) present in tRNA and rRNA, used in RNA modification research, in modified mRNA and as a standard for LC-MS/MS quantification of RNA modifications. Supplied by NorrChemica with DDP shipping. CoA and SDS provided.
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NorrChemica™

5-Methylcytidine

CAS 2140-61-6 ≥97%

5-Methylcytidine | CAS 2140-61-6 | ≥97%

Pack sizes & prices

Pack size Excl. VAT Incl. VAT
1.0 g €54,20
2.0 g €39,90
5.0 g €149,20
10 g €298,20
25 g €649,20
50 g €1.250,00
100 g €1.750,00

Taxes and fees, e.g. VAT, included in the price. Shipping calculated at checkout.Free shipping on EU, UK and EFTA orders above €250, and above €400 to the rest of the world.

Technical Specifications

CAS Number 2140-61-6
EC / EINECS Number 218-390-8
MDL Number MFCD00006548
SMILES CC1=CN(C(=O)N=C1N)[C@H]2[C@@H]([C@@H]([C@H](O2)CO)O)O
InChI InChI=1S/C10H15N3O5/c1-4-2-13(10(17)12-8(4)11)9-7(16)6(15)5(3-14)18-9/h2,5-7,9,14-16H,3H2,1H3,(H2,11,12,17)/t5-,6-,7-,9-/m1/s1
InChIKey ZAYHVCMSTBRABG-JXOAFFINSA-N
PubChem CID 92918
Molecular Formula C₁₀H₁₅N₃O₅
Molecular Weight 257.24 g/mol
Melting Point 213 °C
Solubility Soluble in water, DMSO, DMF and phosphate-buffered saline (pH 7.2).
Purity ≥97%
Physical Form White to off-white solid powder
HS Code 2934.99
Shelf Life Retest period: 36 months from date of manufacture
Storage Conditions Store at −20 °C in a tightly sealed container, protected from light and moisture.

Product Description & Scientific Applications

5-Methylcytidine (m5C) is a modified ribonucleoside.

  • RNA modification. 5-Methylcytidine occurs in tRNA and rRNA in bacteria, archaea and eukaryotes. Transcriptome-wide mapping has also reported sites in mRNA and other non-coding RNAs.
  • RNA methyltransferases. In animals the methyltransferase NSUN2 modifies specific tRNAs. Human DNMT2 methylates cytosine 38 in the anticodon loop of tRNAAsp.
    NOHOHOOHONNH2
    NSUN2, DNMT2
    ⟶
    (in tRNA)
    NOHOHOOHONNH2

    Reaction (in tRNA): cytidine residue → 5-methylcytidine (m5C) residue.

  • Oxidation to 5-hydroxymethylcytidine. Tet enzymes convert 5-methylcytidine to 5-hydroxymethylcytidine in RNA in vitro. 5-Hydroxymethylcytidine is present in RNA of mammalian cells and tissues.
    NOHOHOOHONNH2
    Tet enzymes
    ⟶
    (in RNA, in vitro)
    OHNOHOHOOHONNH2

    Reaction (in RNA, in vitro): 5-methylcytidine (m5C) → 5-hydroxymethylcytidine.

  • Modified mRNA. Replacing cytidine with 5-methylcytidine in mRNA made by in vitro transcription lowers the innate immune response to the RNA and raises protein expression. Modified nucleosides are used in mRNA vaccines for this reason, the approved COVID-19 vaccines using N1-methylpseudouridine.
  • Analytical standard. Synthetic 5-methylcytidine is used to prepare calibration solutions for LC-MS/MS quantification of RNA modifications.

Shipping Destinations

  • EU & UK: Priority delivery, 2–5 business days.
  • United States (DDP): 3–7 business days, duties and taxes prepaid.
  • EFTA Countries (DDP): 3–7 business days, duties and taxes prepaid.
  • Worldwide: 7–14 business days, selected locations.

Safety Information

Hazard Class Not regulated for transport
Transport Category Not classified as dangerous goods for transport (ADR/IATA/IMDG)

Documentation

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