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β-Nicotinamide Mononucleotide

CAS 1094-61-7 ≥99.0%

β-Nicotinamide Mononucleotide | CAS 1094-61-7 | ≥99.0%

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Technical Specifications

CAS Number 1094-61-7
EC / EINECS Number 214-136-5
MDL Number MFCD00038748
SMILES C1=CC(=C[N+](=C1)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)(O)[O-])O)O)C(=O)N
InChI InChI=1S/C11H15N2O8P/c12-10(16)6-2-1-3-13(4-6)11-9(15)8(14)7(21-11)5-20-22(17,18)19/h1-4,7-9,11,14-15H,5H2,(H3-,12,16,17,18,19)/t7-,8-,9-,11-/m1/s1
InChIKey DAYLJWODMCOQEW-TURQNECASA-N
PubChem CID 14180
Molecular Formula C₁₁H₁₅N₂O₈P
Molecular Weight 334.25 g/mol
Melting Point 166 °C (dec.)
Solubility ~50 mg/mL in water (25 °C)
Purity ≥99%
Physical Form White crystalline powder
HS Code 2934.99
Country of Origin Finland
Shelf Life Retest period: 36 months from date of manufacture
Storage Conditions Store refrigerated (2–8 °C) in a tightly sealed container

Product Description & Scientific Applications

β-Nicotinamide Mononucleotide (β-NMN; nicotinamide ribotide; CAS 1094-61-7) is the biologically common β-anomer of nicotinamide mononucleotide, a pyridine nucleotide and central intermediate in nicotinamide adenine dinucleotide (NAD⁺) biosynthesis. It is distinguished from the α-anomer (α-NMN), which differs in stereochemistry and analytical behaviour. In the mammalian salvage pathway, nicotinamide phosphoribosyltransferase (NAMPT) converts nicotinamide and 5-phosphoribosyl-1-pyrophosphate into β-NMN, which nicotinamide mononucleotide adenylyltransferase (NMNAT) enzymes adenylate to NAD⁺. It is supplied as a white to light-yellow powder, soluble in water.

Enzymatic Intermediate in NAD⁺ Biosynthesis Research

NMN is a direct substrate for ATP-dependent, NMNAT-catalysed NAD⁺ formation. Mammalian systems contain three NMNAT isoforms — NMNAT1, NMNAT2, and NMNAT3 — differing in tissue distribution, subcellular localisation, and catalytic properties. It also supports steady-state kinetic and substrate-specificity measurements, and pathway-flux experiments distinguishing salvage-pathway intermediates from downstream dinucleotides and related pyridine nucleotide metabolites.

Biochemical Tool for NAD⁺-Dependent Enzymology

NAD⁺ is consumed by enzyme families including sirtuin deacylases, poly(ADP-ribose) polymerases, and CD38-family ectoenzymes. NMN is used in studies connecting precursor availability with NAD⁺-dependent activity.

Noncanonical Redox Cofactor in Engineered Biocatalysis

Oxidised NMN and reduced NMNH have been developed as a noncanonical redox cofactor pair for engineered enzymatic systems. In cell-free and engineered-cell platforms, the NMN⁺/NMNH cycle can be separated from natural NAD(P)⁺/NAD(P)H pools, letting researchers design orthogonal redox pathways and tune cofactor specificity.

Analytical and Molecular Biology Applications

NMN serves as an analytical reference compound in LC-MS/MS, UHPLC-MS/MS, and HPLC methods quantifying NAD⁺ metabolites and related pyridine nucleotide intermediates. It is polar and chemically labile; analytical workflows attend to chromatographic retention and isomer separation. NMN is also used in RNA aptamer binding studies and ribozyme activation research involving β-NMN-responsive or β-NMN-activated RNA systems.

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Safety Information

GHS Pictograms
Hazard Class None — not subject to transport regulations
Transport Category Not classified as dangerous goods for transport (ADR/IATA/IMDG)

Documentation

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