• Chemical structure of Fast Green FCF (Food Green 3 / FD&C Green No. 3; C.I. 42053), CAS 2353-45-9, 95%— sea-green sulfonated triphenylmethane dye, the disodium inner salt (the form supplied; C37H34N2Na2O10S3, λmax ≈ 625 nm). Used as a protein stain in isoelectric focusing and PAGE, a quantitative histone stain at alkaline pH, and a permitted food, drug and cosmetic colorant (E143). Supplied by NorrChemica with DDP shipping. CoA and SDS provided
  • Fast Green FCF optical/indicator reference card (NorrChemica). CAS 2353-45-9, C.I. Food Green 3 / FD&C Green No. 3, a disodium triarylmethane trisulfonate dye. Shows its structure and peak visible absorption at λabs ≈ 624 nm in water; blue-green solid, green aqueous solution.
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NorrChemica™

Fast Green FCF

CAS 2353-45-9 ≥95%

Fast Green FCF | CAS 2353-45-9 | ≥95%

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Technical Specifications

CAS Number 2353-45-9
EC / EINECS Number 219-091-5
MDL Number MFCD00013053
RTECS Number BQ4425000
SMILES CCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC=C(C=C2)C(=C3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3)C5=C(C=C(C=C5)O)S(=O)(=O)[O-].[Na+].[Na+]
InChI InChI=1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
InChIKey RZSYLLSAWYUBPE-UHFFFAOYSA-L
PubChem CID 16887
Molecular Formula C37H34N2Na2O10S3
Molecular Weight 808.85 g/mol
Melting Point 290 °C (dec.)
Solubility Freely soluble in water (gives intensely green-blue solution); soluble in ethanol and methanol.
Purity ≥95%
Physical Form Sea-green to dark-blue powder
HS Code 3204.12
Country of Origin Finland
Shelf Life Retest period: 36 months from date of manufacture
Storage Conditions Store in a cool, dry place in a tightly sealed container, protected from light

Product Description & Scientific Applications

Fast Green FCF is an anionic green dye and a food colorant, also called Food Green 3, Green 1724, and Solid Green FCF. Its absorption maximum lies between 622 and 626 nm.

Fast Green FCF improves the visualization of the extracellular matrix in ex vivo fluorescence confocal microscopy. Paired with acridine orange as a nuclear stain, it sharpens structural detail, enhances the analysis of fibrosis, and yields morphological detail equivalent to or better than frozen sections in most categories. The rapid protocol suits intraoperative microscopy.

In histology the dye is the green counterstain of the Sirius Red/Fast Green method. Sirius Red stains collagen fibres red and Fast Green stains the non-collagenous components green; the collagen stands out for both qualitative inspection and quantitative morphometric image analysis. The combination resolves even thin collagen networks and, in normal and inflamed colon, detects collagen more sensitively than van Gieson or Sirius Red alone.

The dye also stains histones at alkaline pH after acid extraction of DNA, and stains proteins separated by electrophoresis.

In water-treatment research the dye serves as a model anionic colorant, and its concentration in solution is read by ultraviolet-visible spectrophotometry at 625 nm. A range of low-cost adsorbents, among them de-oiled soya, bottom ash, peanut hull, and coffee husk, remove it from aqueous solution.

Shipping Destinations

  • EU & UK: Priority delivery, 2–5 business days.
  • United States (DDP): 3–7 business days, duties and taxes prepaid.
  • EFTA Countries (DDP): 3–7 business days, duties and taxes prepaid.
  • Worldwide: 7–14 business days, selected locations.

Safety Information

GHS Pictograms
GHS07 Harmful/Irritant
Signal Word Warning
Hazard Class Not regulated for transport
Transport Category Not classified as dangerous goods for transport (ADR/IATA/IMDG)
H-Statements H341
P-Statements P201 - P202 - P261 - P271 - P280 - P304+P340 - P308+P313 - P405 - P501
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