What is Orcein (Synthetic) used for?

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Where can I buy Orcein (Synthetic) in Europe?

Orcein (Synthetic) is available from NorrChemica (Helsinki, Finland) with DDP worldwide shipping. EU and UK delivery in 2-5 business days. All orders include batch-specific CoA and SDS.

What is the CAS number of Orcein (Synthetic)?

Orcein (Synthetic) has CAS number 1400-62-0 and is supplied at Synthetic. Drying loss: ≤25%. Absorbance (E1%1cm) ≥ 295 (MeOH, 579–584 nm). purity.

What purity is Orcein (Synthetic) available in?

Orcein (Synthetic) is available at Synthetic. Drying loss: ≤25%. Absorbance (E1%1cm) ≥ 295 (MeOH, 579–584 nm). purity with batch-specific Certificate of Analysis.

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NorrChemica™

Orcein (Synthetic)

Name: Orcein (Synthetic) | CAS 1400-62-0 | E1%1cm ≥ 295 (MeOH, 579–583 nm)
Brand: NorrChemica
SKU: NOR-1400620-1g
Availability: InStock

CAS 1400-62-0 E1%1cm ≥ 295 (MeOH, 579–583 nm)

Orcein (Synthetic) | CAS 1400-62-0 | E1%1cm ≥ 295 (MeOH, 579–583 nm)

€39,90 EUR (incl. VAT)

€39,90 EUR

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Weight 1.0 g 2.0 g 5.0 g 10 g 25 g

Technical Specifications

CAS Number	1400-62-0
EC / EINECS Number	215-750-6
MDL Number	MFCD00062310
SMILES	CC1=CC(=CC(=C1NC2=C(C3=C(C=C2O)OC4=CC(=O)C(=NC5=C(C=C(C=C5C)O)O)C(=C43)C)C)O)O
InChI	InChI=1S/C28H24N2O7/c1-11-5-15(31)7-17(33)25(11)29-27-13(3)23-21(9-19(27)35)37-22-10-20(36)28(14(4)24(22)23)30-26-12(2)6-16(32)8-18(26)34/h5-10,29,31-35H,1-4H3
InChIKey	QFIIYGZAUXVPSZ-UHFFFAOYSA-N
PubChem CID	72685
Molecular Formula	C₂₈H₂₄N₂O₇
Molecular Weight	500.5 g/mol
Solubility	Variable by batch and pH. Practically insoluble to sparingly soluble in water; soluble in ethanol (0.1–4%)
Purity	Synthetic. Drying loss: ≤25%. Absorbance (E1%1cm) ≥ 295 (MeOH, 579–584 nm).
Physical Form	Reddish-brown powder
HS Code	3204.19
Shelf Life	Retest period: 36 months from date of manufacture
Storage Conditions	Store in a cool, dry place in a tightly sealed container

Product Description & Scientific Applications

Orcein is a phenoxazone dye, a mixture of amino- and hydroxyphenoxazone chromophores resolved chromatographically into fourteen components. Its natural form, orchil, is a lichen-derived purple dye used since the sixth century, and the synthetic dye supplied here is applied as a histological stain.

Elastic-tissue staining. Orcein is a long-established elastic-fibre stain. Applied from acidified alcohol in the Taenzer–Unna method, it stains elastic fibres selectively within the tissue section. Comparative dye trials implicate the aminophenoxazone chromophore in this elastin affinity.

Hepatitis B surface antigen. In Shikata's orcein method the dye demonstrates hepatitis B surface antigen as cytoplasmic inclusions in paraffin sections of liver, staining hepatocyte cytoplasm but not nuclei, including the ground-glass hepatocytes of carriers. The method is specific and inexpensive and develops the inclusions even in stored, formalin-fixed, or autolysed material, which has made it a routine histochemical stain for the antigen.

Copper-associated protein. The same Shikata method stains a copper-associated protein in hepatocyte cytoplasm in long-standing cholestatic liver disease. The orcein-positive granules are rich in protein and sulphydryl groups and co-localise with dedicated copper stains such as rubeanic acid, marking the accumulated copper-binding protein.

Chromosome counts and ploidy. Dissolved in acidified solution as aceto-orcein, the dye stains chromosomes in squash preparations, supporting rapid chromosome counts and ploidy determination.

Polytene chromosome banding. The same aceto-orcein stains the giant salivary-gland chromosomes of Drosophila, resolving the banding pattern that marks individual chromosome regions for cytogenetic mapping.

Plant genotoxicity testing. In the Allium cepa assay, aceto-orcein squashes of root-tip meristems reveal the mitotic index, chromosomal aberrations, and micronuclei that score the genotoxicity of test agents and environmental samples.

Pollen viability. The stain also marks mature pollen, separating viable grains from aborted ones in fertility and plant-breeding assessment.

Historical dye analysis. In its natural form the dye colours textiles, parchments and illuminated manuscripts, and once stood in for Tyrian purple. Conservation scientists identify it on such objects by HPLC-MS, the orcein marker profile also distinguishing the lichen species of origin.