What is Tricine used for?

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Where can I buy Tricine in Europe?

Tricine is available from NorrChemica (Helsinki, Finland) with DDP worldwide shipping. EU and UK delivery in 2-5 business days. All orders include batch-specific CoA and SDS.

What is the CAS number of Tricine?

Tricine has CAS number 5704-04-1 and is supplied at ≥98% purity.

What purity is Tricine available in?

Tricine is available at ≥98% purity with batch-specific Certificate of Analysis.

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NorrChemica™

Tricine

Name: Tricine | CAS 5704-04-1 | ≥98%
Brand: NorrChemica
SKU: NOR-5704041-25g
Availability: InStock

CAS 5704-04-1 ≥98%

Tricine | CAS 5704-04-1 | ≥98%

Pack sizes & prices

Pack sizes and prices
Pack size	Excl. VAT	Add to cart
25 g	€27,25€1,09/g€34,20 incl. VAT
50 g	€39,20€0,78/g€49,20 incl. VAT
100 g	€67,09€0,67/g€84,20 incl. VAT
250 g	€118,88€0,48/g€149,20 incl. VAT
500 g	€223,03€0,45/g€279,90 incl. VAT
1000 g	€421,67€0,42/g€529,20 incl. VAT

Prices are shown without VAT. The price in the box includes VAT at 25.5% for Finland. Shipping calculated at checkout.Free shipping on orders above €250 incl. VAT. For businesses in other EU countries that give a VAT number, the limit is €250 excl. VAT.

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Technical Specifications

CAS Number	5704-04-1
EC / EINECS Number	227-193-6
MDL Number	MFCD00004277
SMILES	C(C(=O)O)NC(CO)(CO)CO
InChI	InChI=1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
InChIKey	SEQKRHFRPICQDD-UHFFFAOYSA-N
PubChem CID	79784
Molecular Formula	C₆H₁₃NO₅
Molecular Weight	179.17 g/mol
Melting Point	187 °C (dec.)
Solubility	Soluble in water (89.6 g/L at 20 °C); insoluble in ethanol and DMSO.
Log P_ow	-2.87 at 25 °C
Purity	≥98%
Physical Form	White crystalline powder
HS Code	2922.50
Country of Origin	Finland
Shelf Life	Retest period: 36 months from date of manufacture
Storage Conditions	Store in a cool, dry place in a tightly sealed container

Product Description & Scientific Applications

Tricine (N-[tris(hydroxymethyl)methyl]glycine; N-tris(hydroxymethyl)methylglycine) is a zwitterionic aminopolyol amino-acid buffer of the Good's series, combining a glycine carboxylate and secondary amine with a tris(hydroxymethyl) headgroup. Its buffering pKa is 8.1 at 25 °C, with an effective range of pH 7.4–8.8, spanning the near-physiological to mildly alkaline window. It is water-soluble.

Buffer chemistry and temperature dependence. Tricine buffers through its secondary-amine group; the pKa falls as temperature rises, while ionic strength has little effect. It has also been characterised as an ampholyte pH-reference standard, determined alongside BES in dimethyl sulfoxide/water mixtures down to subzero temperatures.

Tricine-SDS-PAGE and protein electrophoresis. Tricine-SDS-PAGE separates proteins in the mass range 1–100 kDa and is the preferred electrophoretic system for resolving proteins smaller than 30 kDa. It uses lower acrylamide concentrations than other systems, which facilitates electroblotting — particularly important for hydrophobic proteins. Tricine also serves as a component of non-barbital agarose buffers for immuno- and zone electrophoresis.

Metal coordination. The tris(hydroxymethyl) nitrogen and the glycine carboxylate make tricine a metal chelator. In its copper(II) complex the tricine anion binds Cu2+ as a tridentate O,N,O′ ligand, giving a five-coordinate, approximately square-pyramidal metal centre within a chloride-bridged polymeric chain